Versatile Vibrational Energy Sensors for Proteins

Abstract Vibrational energy transfer (VET) is emerging as key mechanism for protein functions, possibly playing an important role dissipation, allosteric regulation, and enzyme catalysis. A deep understanding of VET required to elucidate its in such processes. Ultrafast VIS‐pump/IR‐probe spectroscopy can detect pathways proteins. However, the requirement having a donor sensor installed simultaneously limits possible target proteins sites; increase their number we compare six IR labels regarding utility sensors. We these terms FTIR, signature donor‐sensor dipeptides different solvents. Furthermore, incorporated four PDZ3 assess capabilities more complex systems. Our results show that be used interchangeably, allowing free choice right label depending on system under investigation methods available.